Ethylene Oxide residuals determination by GC-HS

Discussions about GC and other "gas phase" separation techniques.

2 posts Page 1 of 1
Hi everyone,

Anyone experienced in determining ethylene oxide residuals in medical devices after sterilization according to ISO 10993-7?

I am currently trying to validate a method for this determination but I have some difficulties:
I decided to purchase ethylene oxide solution (certified reference material) to make the calibrations but I have a lot of difficulties defining my peak of interest in the reference solution (which is not normal) .. I believe that the chromatographic parameters are not the most appropriate and also knowing that ethylene oxide is highly volatile ...

My idea will be to optimize the method for the determination of ethylene oxide present in aqueous extracts resulting from the extraction of medical devices in water. I wanted to make this determination by headspace gas chromatography.

Anyone with experience who can give some tips for this early phase of method optimization?


Help please!!

Thank you very much
As far as I remember while working at BD Medical.
First EO residue Extraction of medical device in DI H2O after it comes out of sterilization chamber
At different interval of times. Like 24 hours, 48 hours 72 hours etc and filling the GC vials and capping the vials with aluminum seal. Next
To match the matrix of the sample
EO standards (USP) prepared in DI H2O
And tested on Agilent GC-MS using waters empower
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry