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- Posts: 14
- Joined: Mon May 13, 2019 11:39 am
I'm a "newbie" with chromatography, and i've had the very first big issue that i can't fix right now. Because of this, i would like to ask for some advice from experienced users.
Well, my problem is that since the new coloumn installation, the response from any samples (standard, sample etc.) is too much for the detector, causing flat topped peaks, unquantificable results. I switched the input to range 10, what caused quantable peaks, but the linearity and stability isn't perfect (and I was still slightly worded). There are two coloumns registered, one is the front ("the bad") and the other is middle for confirmation analysis. The main issue is that my "quantification" line is so bad, that i haven't been sure if the values are correct, since the coloumn installation. (before it, everything was right). I've read somewhere that my ECD cell may contaminated, and that is why i cant get normal chromatogram. (The coloumn installation was not correct either, because we weren't capped the ECD inlet)
Other issue: I have ghost peaks from previous sample runs (standard peaks overbleeding to the next runs), but it is only occuring on the FRONT coloumn, the middle isnt bleeding like this.
If anyone have some advice, or could help with telling same solved problem experiences, would be super.
Thanks!