-
- Posts: 82
- Joined: Tue Nov 27, 2012 11:26 am
I'm trying to derivatize a standard solution of acetic acid and ethylene glycol in acetonitrile (ACN). I'm taking 100 uL of the solution and 5 uL of BSTFA w 1%TCMS into a 20 mL headspace vial and heating at 80 C for 15 min before injecting. I ran a blank, 100 uL of ACN and 5 uL BSTFA w TCMS, and saw 3 large peaks in addition to the ACN peak. Here is the chromatogram.
Would you expect to see the 3 large peaks from the BTSFA w TCMS? I do not have any reference method or chromatogram to compare to and I haven't done this before. Just to make sure the peaks are not coming from the ACN, I injected just 100 uL of ACN. Here is that chromatogram. The 3 peaks are not preset.
Thanks for your replies,
Dave