Hi,

The general chapter 2.4.25 of the EP has the following requirement for the Verification of precision:
For each pair of injections, calculate for ethylene oxide and for dioxan the difference in area between the peaks obtained with the test solution and reference solution (a). The test is not valid unless the relative standard deviation of the 3 values obtained for ethylene oxide is not greater than 15 per cent and the relative standard deviation of the 3 values obtained for dioxan is not greater than 15 per cent. If the weighings used for the test solution and reference solution (a) differ from 1.00 g by more than 0.5 per cent, the appropriate corrections must be made.

Has somebody made these corrections? I have weights that differ from 1.00 g by more than 0.5 percent and I have no idea how to apply a weight correction to the calculation required above. Two things have to be made, the difference between the peaks in the test solution and the reference solution, and then an RSD of the 3 replicates performed of test/reference solutions. Where would a weight correction apply here?