TPH concentration with GC-FID

Discussions about GC and other "gas phase" separation techniques.

3 posts Page 1 of 1
Hello, I'm trying to evaluate the TPH concentration of the soil sample(diesel contaminated) using the GC-FID.

To test the GC method and condition, I purchased TPH reference reagents with 17 alkane components(C8-C40) in it, all same concentration (500 ppm).

I got clear 17 peaks, and each of their areas is similar around a value of 300.

Next step is to measure the "real sample", but now I run into difficulties.

Since I used the TPH reference reagent for the GC test, I have no information (retention time, area...)about other compounds in the diesel such as PAHs...

Here are my questions:

1) How can I determine the TPH (Total Petroleum hydrocarbon) concentration, unless I get information about all the peaks for the compounds in the diesel? Now I got only 17...

2) I got 17 peaks with the same value of area around 300, with the reference reagent (500 ppm, C8~C10 alkane). Can I assume that other components in diesel within that range also show similar peak? (Area 300 for 500 ppm)

I just want to know the TPH concentration, not each of them respectively.

I'm waiting for help of someone who has measured the TPH concentration with the GC-FID. I'm using HP-5 column (not HP-5ms) :(
There's guidance in this forum about hydrocarbon range calibration. Also check out methods 8015, 8100, 8000, and CT ETPH for more. Your calibration table needs to have an entry for the summation of all those 17 peaks, then when running an unknown, integrating from before C8 to just after C40 will give you your TPH value.

or contact me via my email
Freek Varossieau
Agilent Technologies
3 posts Page 1 of 1

Who is online

In total there are 14 users online :: 1 registered, 0 hidden and 13 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: Baidu [Spider] and 13 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry