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- Posts: 56
- Joined: Tue Feb 06, 2018 10:38 pm
I have a DB-Wax column. The column had been baked 3 times. First time was trimmed and baked overnight. 2nd time was full maintenance, timmed and baked (no bleeding occured). Third time full maintenance, trimmed, and baked overnight again at 180 degrees. Then i ran some straight water and big column bleed starting occurring i am not sure that is the column destroyed?
We have an oxygen and moisture trap installed for the carrier gas helium. We did inject plant sample mixed with water, filtered and shoot. Now the column is bleeding too much. Can anyone please help what i can do to lower the bleed. Thank you in advance picture is also attached.
https://cdn.pbrd.co/images/HyZYSEW.jpg