by
Hollow » Fri Jul 13, 2018 4:58 pm
Murshad wrote:
Thanks for your inputs.
Actually I want to degrade the column to the level that plate count decreases to half. Changing the column dimensions or any modification in any operational parameter is not an option.
Thanks in advance
I don't understand why one would degrade a column by purpose to get less plates?
If you have plates in excess you may trade them in for getting things done faster (higher flow rates or use shorter column).
But that seems not to be what you want.
other ideas: start overloading your column or use a suboptimal injection solvent/settings or make lousy connections to get your peaks broader... [/silly mode off]