by
Kaliane » Mon Jun 04, 2018 8:56 pm
Hello!
I'm using a Master GC (model) by Dani (brand). The lenght of the liner is 7 cm and the diameter is 4 mm. I'm using split mode. I'm injecting 1 microliter.
I'm using a capillary column, with polyethylene glycol as
stationary phase. (50 x 0,25 x 0,2).
I changed the septum recently. I also tried reconect the column and changed the ferrule and the glasswool.
The temperature starts at 60°C during 2 minutes, the rate is 15°C/min until 120°C (maintain for 3 minutes) and then the rate is 50°C/min until 200°C (maintain for 1 minute). The flow is 2 mL/min. Split ratio 1:100. The carrier gas is nitrogen. The detector is a FID.
The analyte is methanol and I'm using 4-methyl-2-pentanol as internal standart. The samples are wine. Before to inject, the samples are distilled.
However, I changed nothing in this parameters... The only thing that I noticed that is changing is the flow's split ... I can't keep it constantly... It is setted to 200 mL/min, but it keep changing from 199 until 201...
Thank you!