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- Posts: 264
- Joined: Sun Nov 14, 2010 2:20 pm
Because of that I don't have feel for how long a retention calibration is typically good for on GC...
But now I find myself in a situation where I have to rely on published retention indices for compound ID (and there can be hundreds of compounds!!!) for high probability compound ID, so the "calibration" is done using n-Alkanes to have the calculate the retention indices
In this case the run times are "VERY" long and I get a LOT of samples. So much so that it is hard to keep up, so the less I can run n-the alkane standards the better...
Mu question is what would be the longest reasonable time between running the alkane standards ...
This is R&D support for early stage process development, so there are no specific QC/QA requirements and the chromatograms can be very different with each experiment...
I do have more to go on than retention index, but a good retention index would really help nail things down... I do have an MS on the GC, but MS often does not ID the right isomer even for large peaks, and has a lot of trouble IDing the small peaks correctly... The MS hits ALONG with retention index would make the IDs much more reliable.
Thanks for any info.
- karen