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- Posts: 7
- Joined: Fri Sep 22, 2017 6:01 pm
Because it is USP method, there is not much room we can change. We contacted FDA, and was told they can easily achieve the required %RSD (0.04%-0.13%). Could you please provide some suggestion of how to improve the reproducibility? Any suggestion is welcome.
Here is the condition:
Injection port: 260°
Detector: 280°
Column: 135°
Carrier gas: Helium
Flow rate: 1.0 mL/min
Injection volume: 1 µL (use 10 uL syringe)
Injection type: Split injection, split ratio 10:1
Run time: 12 min
column is 0.32-mm × 30-m fused silica, 0.25-µm DB-wax (brand new column)
liner: Restek split liner with wool
Septum purge flow: 3 mL/min
injection speed: 500 uL/sec
Thank you!