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- Posts: 7
- Joined: Tue Sep 12, 2017 12:45 pm
I am using a Gas chromatograph (CP-3800) with a FID detector and a capillary column (VF-5 ms, 30 m × 0.25 mm with 0.25 μm film thickness of 5% phenyl-methylpolysiloxane column) to analyse fatty acid methyl esters (FAMEs).
For the analysis, 1 ml of total sample volume is prepared using 100 µL of FAMEs + 20 µL of internal standard + 880 µL of heptane. 1 µL from this 1 ml is injected into the column with a split ratio of 100.
However, split injection is not working properly now. Thus, i am thinking to do the injections using splitless mode by doing the manual dilution instead of on column dilution.
can somebody suggest me, how to prepare the sample of the same composition (same as with split ratio of 100) to do the injections with splitless mode.
Thank you!