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- Posts: 53
- Joined: Mon Sep 26, 2011 4:02 pm
- Location: USA
Thanks for all replies.
Discussions about GC and other "gas phase" separation techniques.
dblux_ wrote:
The best answer you can get exactly tailored to your specific compounds and method parameters would be if you compare chromatograms with current inlet temperature against chromatogram with increased inlet temperature by 20-30 deg.
lmd_not wrote:
Hi!
I have also been some trials this week with inlet temperature. In my case, I tested 210, 250 and 290 ºC. I am trying to quantify amphetamine and it is a thermolabile compound, so we started with 210 ºC...without taking into account that its boiling point is 203 ºC .
210 is so close to the bp that the peaks of the first levels of calibration were pretty small (S/N<10) and the repetibility of the areas was very bad.
Then we tried 250 and the areas were much better.
At 290, the repetibility of the areas at some points of the curve, mainly the most concentrated, was very bad, both for the analyte and for the IS... so I guess that perhaps something happened at that temperature. Finally, I think we will chose 250ºC since it seems to give the most optimal results.
I hope that helps... I'm not an expert at GC, learning every day and coming to conclusions from my results but with any expert to confirm them I guess at the end it is about trying and see what give you the best results.
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