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- Posts: 82
- Joined: Fri Oct 13, 2006 6:13 pm
I'm reading that typically centrifugation is used in order to purify the yeasts and enzymes, but is centrifugation a good enough method to get everything out of the mixture to be analyzed? Would the enzymes produced by the yeasts also be taken out like the yeasts/molds?
Just want to avoid anything that could be detrimental to our instruments or columns. Most of the stuff I end up finding if the opposite of what I'm looking for (looking at the yeasts/enzymes, not the reaction they catalyzed). If we are not interested in those components, but only in the small organic molecules, is simple filtration over silica enough?