Not at all familiar with this type of sample. I have a student who runs enzymatic reactions (amine couplings of small molecules) using the enzymatic activity of things like yeasts and molds (candida, aspergillus, Lipase-B, etc.) and they would like to perform analysis by GC/FID and GC/MS.

I'm reading that typically centrifugation is used in order to purify the yeasts and enzymes, but is centrifugation a good enough method to get everything out of the mixture to be analyzed? Would the enzymes produced by the yeasts also be taken out like the yeasts/molds?

Just want to avoid anything that could be detrimental to our instruments or columns. Most of the stuff I end up finding if the opposite of what I'm looking for (looking at the yeasts/enzymes, not the reaction they catalyzed). If we are not interested in those components, but only in the small organic molecules, is simple filtration over silica enough?