-
- Posts: 4
- Joined: Fri Sep 17, 2021 5:50 pm
I ran residual solvent curve at high concentration (1500ug to 150ug off instrument), there was no linearity. I then ran a curve using liquid injection, the curve was almost perfect. So there's something going on with my headspace. From looking at the curve, it looks like there's some kind of saturation at higher concentrations.
I then ran a 3-point curve with lower concentrations (240 to 60). Way better linearity, but still not great. I’m also noticing that at higher concentrations, the ethanol peak is way smaller than it’s surrounding compounds. At lower concentrations, the ethanol peak is higher in comparison to the surrounding compounds.
I don’t believe this is detector saturation, but something else is going on because: The peak shape is showing no signs of saturation (peak fronting or flat tops). Also, running at a higher split ratio does not seem to help. So my question is, why is this happening?
Here are my current run conditions:
Inlet:
Temp: 150
Split: 50:1
4mm Straight Topaz Liner
Column:
Constant flow at 2.0 ml/min
Hydrogen gas
Oven:
33, hold for 6
15 80 0
55 250 1
Detector: 320
Headspace (CTC PAL):
Injection Volume: 500uL
Incubation temp: 80
Incubation Time: 1800
Syringe Temp: 110
Injection Speed: 500
I couldn't attach my pictures, but I also posted this on the Agilent forum with pics
https://community.agilent.com/technical ... -happening