GC column flow

Discussions about GC and other "gas phase" separation techniques.

5 posts Page 1 of 1
Hello,
What is the best flow for a column with the following dimensions?
30m*0.25mm*0.25μm

Thank you
I would start from 1.5 mL/min, but it could be between 1.2 and 1.8 mL/min, depending on your application
Thank you trozen
Optimum flow (giving the best resolution) depends on the carrier gas in use. With hydrogen & helium faster flows can be used with relatively little change in resolution, but column efficiency drops of fairly rapidly below the optimum.

As a rough guide a linear velocity of 10 cm/s is near optimum for nitrogen, helium's optimum is roughly twice this (20cm/s) & hydrogens near double again (40cm/s).

Look up 'Van Deemter' plots, for a fuller picture.
5 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry