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- Posts: 148
- Joined: Wed Jul 03, 2013 1:51 am
- Location: Michigan, USA
I am introducing retention index to our lab to reduce the fraction of false ids. I got 1000 ug/mL C7-C30 alkanes in hexane from Sigma.
My plan is to dilute 1 in 100 in n-hexane to have 10 ppm of each analyte. Do you agree with this approach. I am planning to stockpile GC vials with this solution and use them for the construction of RI calibrations on our GC-FID, GC-TCD, and GC-MS systems.
A few concerns:
1. hexane will elute when the MS is already on. I do not really want to have lab techs make changes to the solvent delay time. How bad is it really for the source to analyze the solvent peak?
2. Would you use DCM or pentane instead for dilution?
3. Should we spike with dodecane to 100ppm or something to get another "anchor" landmark in the chromatograms in order to avoid "one-off" errors?
4. Is 10 ppm an appropriate concentration for GC-FID and GC-TCD systems? I know it is plenty for GC/MS?
Thanks!