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- Posts: 2
- Joined: Fri Jun 18, 2021 2:52 pm
I'm new in this forum. I am a PhD student and I have a problem with evaluating some chromatograms. I hope you can help me.
I'll explain the problem: I have to analyze a sample in which there is a carboxylic acid, a lactone and the 4-hydroxy pentanoic acid. Not having the standard of 4-hydroxy pentanoic acid and having two very close peaks, I'm not sure of good separation.
The lactone comes out at 9.02min and the hydroxypentanoic at 8.63min.
given the situation I can definitely confirm that 8.63 is the hydroxy and not the lactone?
Thank you so much