Hi, can someone help me, please, I am working with the determination of FAMEs in fish oil, I have the standard of FAME 37 and PUFA 3, I use the HP-88 column of 60 mts x 0.25 ID, I can separate All the 37 FAME compounds of the supelco standard and I identify them in the calibration table of the VARIAN Star and with the Agilent Chemstation software which I am using, but my problem is when I run the FAME PUFA 3 standard, the C20:1n9 compound identified in PUFA 3 does not coincide with the compound indicated in the PUFA 3 certificate, while the peak indicated in the certificate as C18:4n3 is not identified as it does not exist in the fame 37 standard, in addition this peak appears as the highest concentration than C20:1n9, which does not coincide with the order of avoidance when comparing my run with that of the PUFA 3 certificate issued by the supplier of the standard. If it possible may column HP-88.

It is possible that my HP-88 column reversed the order of elusion of the peaks and that the C20: 1n9 eluted first than the C18: 4n3, is this possible? Can someone help me to correctly identify the PUFA 3 standard, please.
I attach a chromatogram of how it is identifying the PUFA 3 standard. It is different from the PUFA 3 certificate seen on the web.

I attach files with the chromatograms:

Download these files:

https://app.box.com/s/xyskyv6jobdk1k070bc18dad80vfcnlu

https://app.box.com/s/sfb7xsp9fgkesn165q0y78tivexoivws

look at the chromatograms