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- Posts: 7
- Joined: Sun Apr 12, 2015 7:20 pm
I am trying to see what effects the relative response factor (RRF) of the FID on a GC-FID. I have purchases a gravimetric standard containing n-C10 to n-C50 that I use to measure the RRF with respect to n-eicosane (one of the components in the standard). The RRF increases dramatically for the final component n-C50. The standard is dissolved in carbon disulfide, an effort is made to make sure all the heavier components in the standard are completely dissolved in the solution prior to injection (the vial is warmed-up prior to the injection on the autosampler), but I still suspect the C50 is falling out of solution within the GC syringe prior to injection, and I believe this to be the cause of the RRF increase.
I would like to give you some details about the GC setup; if need be I can upload the entire method parameters. The method uses a COC inlet and a Restek MXT- HT simdist column (https://www.fishersci.com/shop/products/restek-mxt-1ht-sim-dist-metal-siltek-treated-capillary-columns/06713284).
Any help on this subject would be appreciated. I am unable to find any papers/articles regarding RRF dependence on method parameters such as FID flows and temperatures, inlet temp etc.