7890B Agilent NO Peaks

Discussions about GC and other "gas phase" separation techniques.

4 posts Page 1 of 1
Hi All, I am having a heck of a time troubleshooting my problem. My 7890B is not registering any peaks (except for the ignition peak). My column is a DB-WAX 530. What I have tried:
Replace Septum, Inlet filter, entire Inlet bracket assembly, Jet, and column.
I have baked the column, I have rinsed the column, I have recut and reinstalled the column.
I have manually injected sample, I have cleaned the syringe, I have confirmed it is drawing sample.
I have confirmed that gas there is no plug in the column, the gas is getting through beautifully.
I get baseline changes at different temperatures and there is a detection upon ignition, so it should not be the detector.
It ignites easily without issue.
I have checked all the lines for leaks.

I am at a complete loss as to what else it can be.
Help?
What are you injecting ? What is the column head pressure , split ratio , injector temperature etc ?
you need more data to let us know where the problem is.
If it were me, I would try a couple of other things at this point. 1) I would shoot some organic vapor, acetone headspace or what not, at the inside of the chimney of the FID. Should see a big signal if the FID is fine.

Next, I would remove the column from a cool inlet and inject some solvent directly onto the head of the column (as best as possible). Then re-connect the column and turn the oven up. Look for peak.

This is where I would go next....

Best regards,
4 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry