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- Posts: 5
- Joined: Wed Mar 11, 2020 2:54 pm
In the running sequence from one injection to the next we got irregular peak sheap (e.g. tailing or shoulder), but in the further injection the peak shape becomes ok again without any intervention. As this anomaly is random in the sequence, if we meet it at solution which contains more components, all peaks show more tailing than normal peak shape or the peaks have shoulder.
Has somebody any idea what can cause the problem? We think that the problem comes from the injection part of the method, but we do not know the exact reason. The injection volume is high - 1 ml, and we use 6%cyanopropylphenyl-94%dimethylpolysiloxane, 30 m, 0.53 mm and 3µm column.
Many thanks for your answer.