Interpret chromatogram and integration intervall

Discussions about GC and other "gas phase" separation techniques.

2 posts Page 1 of 1

I am a beginner in gas chromatography and I still have difficulties with the interpretation of peaks in the chromatogram.

My question on this would be, how can I interpret this measurement from my standard.


What can the smaller peak be and how should I integrate?

And what I also still don't understand is, why does the base line rise so much, after the peak of the solvent?
You have given nowhere near enough information to allow specific answers, but in VERY general terms:

1. the mystery peak could be anything:
- a degradation product from your standard ?
- a contaminant in your standard or your diluent ?

2. the rising baseline is most probably associated with the increase in temperature (assuming you are temperature programming, not isothermal):
- some detectors are more sensitive to temperature effects than others
- some stationary phases are more volatile than others and "bleed" as the temperature increases.
-- Tom Jupille
LC Resources / Separation Science Associates
+ 1 (925) 297-5374
2 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry