Glycerol Tricaprylate

Discussions about GC and other "gas phase" separation techniques.

3 posts Page 1 of 1

I was wondering if anyone has had any experience performing the content of triglcerides test using the USP method for glycerol tricaprylate.
I test Neobee/medium chain triglycerides on a normal db5MS column. They come off at 300-325 and show up as 4 main peaks glycerol (C8)3, (C8)2(C10)1, (C8)1,(C10)2, and (C10)3
I'm testing using GC-FID. My the solution I'm having problems with is a 20mg/ml solution of 1-octanoyl-rac-glycerol + 1-decanoyl-rac-glycerol in THF.

I'm getting very shark fin like chromatography and I'm wondering if this is normal especially as the column is rather small.

I'm using an agilent DB-5HT column with dimensions 10m x 0.32mm x 0.1um. for a 1ul injection with a split of 50:1. As its a pharmacopeia method I don't have much leeway to adjust the method parameters.
3 posts Page 1 of 1

Who is online

In total there are 13 users online :: 0 registered, 0 hidden and 13 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 13 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry