Derivatization with MSTFA, NH4I and Ethanethiol

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

4 posts Page 1 of 1
Hi all. I need to follow a method that involves derivatization using MSTFA, NH4I and Ethanethiol prior to GC-MS.

Does anyone know if these reagents are suitable for injection into a GC column (HP-1 or HP-5 columns) or do they really need to be blown to dryness?

If I do want to dilute further, can anyone recommend a suitable solvent for this? Hexane perhaps?

Many thanks.
Methinks you need to re-word and add more information before I can comment.
short answer: yes, you can inject directly onto GC column

for dilution you may use toluene but keep in mind that some TMS derivatives may not be stable in the absence of excess reagent
Looking at the list of reagents I don't see much of a problem - unless there is some thing in the sample that could generate a problem. If your method will stand a packed GC inlet liner - I would recommend that to keep junk off the head of the column. And in the worst case, if the column is degraded, I expect it to be a slow process - and a column is a consumable item. I've seen where people spend many man hours hours cleaning up samples to save on the cost of a column. There comes a point...
4 posts Page 1 of 1

Who is online

In total there are 6 users online :: 0 registered, 0 hidden and 6 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 6 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry