Derivatization with MSTFA, NH4I and Ethanethiol

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

4 posts Page 1 of 1
Hi all. I need to follow a method that involves derivatization using MSTFA, NH4I and Ethanethiol prior to GC-MS.

Does anyone know if these reagents are suitable for injection into a GC column (HP-1 or HP-5 columns) or do they really need to be blown to dryness?

If I do want to dilute further, can anyone recommend a suitable solvent for this? Hexane perhaps?

Many thanks.
Methinks you need to re-word and add more information before I can comment.
short answer: yes, you can inject directly onto GC column

for dilution you may use toluene but keep in mind that some TMS derivatives may not be stable in the absence of excess reagent
Looking at the list of reagents I don't see much of a problem - unless there is some thing in the sample that could generate a problem. If your method will stand a packed GC inlet liner - I would recommend that to keep junk off the head of the column. And in the worst case, if the column is degraded, I expect it to be a slow process - and a column is a consumable item. I've seen where people spend many man hours hours cleaning up samples to save on the cost of a column. There comes a point...
4 posts Page 1 of 1

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