-
- Posts: 3
- Joined: Thu Sep 12, 2019 9:30 am
The question is which solvent or solvent mixture might be suitable to pull the compound with such lower logP from the aqueous phase?
Discussions about sample preparation: extraction, cleanup, derivatization, etc.
Pynball wrote:
We are comparing two different approaches to sample prep, liquid-liquid extraction vs. SPME, but with a derivatization reagent different from the one in the publication.
transfers Amiens
Initially a derivatization is done, then an extraction solvent is added, shaken, centrifuged and then organic layer was evaporated to dryness in a vial. Water and salt are added and SPME is performed. It has nothing to do with on-fiber derivatization.
The sample is urine, and some volatiles which are present in certain diseases are measured.
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.