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- Posts: 12
- Joined: Thu Sep 12, 2019 3:59 am
I've been working on method development for a molecule and the sample matrix is pH=7.4 buffer with salts. The QC samples have been behaving very strange. All the QCL samples recovery is around 80%. But the QCH samples recovery is sometimes 93%, sometimes around 65%. I've tested for several times and I just cannot control it.
I tested the stock solution from which I prep the QC samples and it's right on spot. I don't know what is happening I've been stuck here for a long time.
I just started doing method development and I really appreciate it if any one can give me some help.