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- Posts: 30
- Joined: Tue Oct 24, 2017 1:30 pm
I am facing some issues with the sample preparation part for analysing acetaminophen in water using LCMSMS. When using the OASIS HLb 6cc (200mg) cartridge for extracting acetaminophen from 200mL of spiked water sample , the recovery of acetaminophen is very Low reaching around 10%.
I had tried using another cartridge mainly Agilent BondELut cartridge but the recovery was also around the same .
I am using EPA1694 as a basis for the analysis and some references from APHA 6810.
Can someone please help me regarding the Low recovery?
I was using vacuum to pass the 200mL sample through the cartridge at a rate of 5-6mL/min.
Prior to extraction of the sample, I had conditioned the cartridges with 20mL of methanol, 6mL of reagent water and 6mL of reagent water at pH2.0 .
The analyses were then extracted into 12mL of methanol.
Thank you for any help provided .
Regards
Hts123