pumpedupchemist wrote:
Methanol is considered the best solvent for both washing and elution due to the consistency of the interactions between both sorbent bed and analyte during elution.
In regards to elution, general rule of thumb is that polar target compounds will elute best with polar solvents; methanol > acetonitrile> ethyl acetate>acetone> THF
thanks for your reply.
is there any relation between efficiency and price when it comes to methanol?
is it possible that so many protocols based on methanol because simply is there a lot of experience and easily available?
i have a protocol that washes with methanol, but elutes with ethyl acetate. im curiosed to try to wash with solvents between methanol and acetate ethyl strength (like acetonitrile.. acetone.. ethanol..). wondering if original protocol didnt do it because it was good enough.. or if there are other reasons for not to use such solvents (like price.. efficiency..)