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- Joined: Mon Sep 02, 2019 12:27 pm
The blank I'm using is spiked with same quantities of both analyte and deuterated analog, then derivatization is performed followed by SPE.
However, when I run the extract on a GC-QQQ, the integral of the deuterated derivative peak is approximately 50% lower than the one belonging to analyte derivative.
What might be the reason for that and how to avoid it?
In my opinion it's unlikely H/D exchange takes place, since all the deuteriums in the analog are alkylic, and the only remaining protons (H) sit at two -OH groups.