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- Posts: 76
- Joined: Tue Oct 05, 2004 10:41 am
- Location: Lugo, Spain
I need your ideas and help with the analysis of acrylamide in potato chips by HPLC-MS/MS. I managed to have an acceptable chromatogram with an Zorbax RX-C8 employing an isocratic method with 98% of acidified water (1% of acetic acid). However, I am not able of finding a suitable extraction protocol which allow me to achive limit of detection of 300 ug/kg set up by teh European legislation. I have tried diferent extraction methods, with cartridged without cartridges, ....
I have also derivitized the acrilimadide, this method was very succefull as it increase considerably de signal however I detected acrilamide everwhere .... I don't know if it is because i used plastic bottles and plastic pipettes.
Lookign forward for your ideas and suggestions