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- Posts: 42
- Joined: Tue May 12, 2009 3:39 pm
- Location: MA, US
We're taking our SPE protocol to sea. I want to avoid shipping hazmat to Bermuda ($$$) so I'm hoping we can precondition our SPE cartridges ahead of time and avoid taking bottles of solvent with us...
here's our protocol, using Oasis HLB cartridges:
Precondition with ethyl acetate, methanol then 0.1% HCl.
Pull through seawater samples, flush with 0.1% HCl and store in freezer until back on land for elution and LCMS.
It's at this point that I realize I'm not entirely sure what the point of preconditioning is? is it just a solvent rinse or is it something more? since the last step is 0.1% HCl, which is non-hazardous and much easier to ship can I simply repeat the 0.1% HCl precondition on-board the ship and 'freshen up" the columns before I pull the samples through?
thanks
Helen