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- Posts: 4
- Joined: Wed Feb 28, 2018 4:09 pm
In short, we dissolved one troche in 200mL of progesterone mobile phase (55 reagent alcohol to 45 water), sonicated before filling to volume, and performing filtration (only using the last 6mL of total 10mL filtered) and dilution when needed. We're using a Waters C18 Nova-Pak 3.9x15mm 4mcm particle size and ran our samples at 245nm - RP-HPLC-PDA but no QDa used yet.
Our average percent yield over the course of a week of testing is 66%.
So I tried making the mobile phase 50:50 and ran the samples at 245nm and 254nm. The calibration curve for each method (A,B,C) was r^2 = 0.9995 each time on average.
We're also going to switch from distilled water to LC-MS grade water and have ordered new guard columns...potentially the last samples in the past could have clogged the column or dirtied it before I even started working here?
We also found this paper https://www.ncbi.nlm.nih.gov/pubmed/15595573 that says to actually melt the suppository (they use a dosage form that has a similar matrix to our troches) before dissolving it. Do you think melting will release that progesterone from the matrix?
Looking for other ideas as to why we're not seeing 90-110% yield of progesterone from compounded glycerin-gelatin-water troches...