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thiourea preparation for HPLC

http://www.chromforum.org/viewtopic.php?f=19&t=81394

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thiourea preparation for HPLC

Posted: Sat Jan 27, 2018 8:51 am
by uhaserge
when performing Biopartitioning Micellar Chromatography which is a branch of Micellar Liquid Chromatography , retention factor is extremely needed.

I need to calculate dead time T zero using thiourea as unretained compound by the column , and then, find retention factor value for my sample.

Please, indicate me how to prepare thiourea for measuring actual void volume of a specific HPLC column ?

Serge

Re: thiourea preparation for HPLC

Posted: Sun Jan 28, 2018 4:30 pm
by tom jupille
The same way you would prepare your actual samples.

Re: thiourea preparation for HPLC

Posted: Mon Jan 29, 2018 1:01 am
by uhaserge
@Tom do you mean with the same concentration ?
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