thiourea preparation for HPLC

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

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when performing Biopartitioning Micellar Chromatography which is a branch of Micellar Liquid Chromatography , retention factor is extremely needed.

I need to calculate dead time T zero using thiourea as unretained compound by the column , and then, find retention factor value for my sample.

Please, indicate me how to prepare thiourea for measuring actual void volume of a specific HPLC column ?

Serge
The same way you would prepare your actual samples.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
@Tom do you mean with the same concentration ?
3 posts Page 1 of 1

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