thiourea preparation for HPLC

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

3 posts Page 1 of 1
when performing Biopartitioning Micellar Chromatography which is a branch of Micellar Liquid Chromatography , retention factor is extremely needed.

I need to calculate dead time T zero using thiourea as unretained compound by the column , and then, find retention factor value for my sample.

Please, indicate me how to prepare thiourea for measuring actual void volume of a specific HPLC column ?

Serge
The same way you would prepare your actual samples.
-- Tom Jupille
LC Resources / Separation Science Associates
tjupille@lcresources.com
+ 1 (925) 297-5374
@Tom do you mean with the same concentration ?
3 posts Page 1 of 1

Who is online

In total there are 2 users online :: 0 registered, 0 hidden and 2 guests (based on users active over the past 5 minutes)
Most users ever online was 599 on Tue Sep 18, 2018 9:27 am

Users browsing this forum: No registered users and 2 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
Follow us on Twitter: @Sep_Science

Liquid Chromatography

Gas Chromatography

Mass Spectrometry