-
- Posts: 6
- Joined: Thu Jun 02, 2016 8:38 am
Does anyone here have an opinion on this and maybe some references?
Discussions about sample preparation: extraction, cleanup, derivatization, etc.
rb6banjo wrote:
The only thing I can think of is what you cast in your allusion. Someone "once-upon-a-time" studied it and determined that somewhere slightly north of 8%, they found that there was a measureable difference in how the matrix behaves in the analysis.
The other consideration might be the dilution factor. Adding 8% MeOH changes an analyte concentration that is really 50 ppm to 46 ppm. Could it be, that's within the error of the measurement and someone didn't want to worry about dealing with dilution factors?
A little more detail on what you're actually trying to accomplish in your analysis might help you get better answers than what I've provided here.
dp_kloos wrote:
In our lab, we adhere to the rule that we do not spike samples (wholeblood) with methanolic standards by more than 8% of the samplevolume. This is mostly in order to keep the matrix as similar as possible, however nobody knows where the 8% is based upon. After some reading you find that people use different mixima for this, ranging from 5-10%.
Does anyone here have an opinion on this and maybe some references?
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.