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I'm new to GC, i have bought a Varian 3800 with FID, I am just wondering if the Essential oils need dilution or they can be used as they are in the GC process?
Thanks,
/Alvin
Discussions about sample preparation: extraction, cleanup, derivatization, etc.
Peter Apps wrote:
Depending mainly what column you have in the GC you cannot put any more than 100ng of any component onto the column (end even that is pushing it). So you need to dilute 100:1 and use a split ratio of 20:1 assuming you have 5 major components in the oil. The more complicated the oil, the less you need to dilute.
Peter
ant78se wrote:Peter Apps wrote:
Depending mainly what column you have in the GC you cannot put any more than 100ng of any component onto the column (end even that is pushing it). So you need to dilute 100:1 and use a split ratio of 20:1 assuming you have 5 major components in the oil. The more complicated the oil, the less you need to dilute.
Peter
Thanks Peter,
The column is 10m long. I wonder if it works if I dilute first the essential oil in 99,5% alcohol and then dilute again with distilled water?
I'm using a Varian 3800 with autosampler.
Don Shelly wrote:
It's best to choose a highly volatile solvent. The solvent needs to "flash" in the injection port and also precede your analytes through the column so as not to interfere with the resolution of your analytes. That's why I recommended DCM. Essential oils should be miscible in DCM and DCM is very volatile and less likely to be retained by your stationary phase.
I agree with Peter that 10 meters is a very short column if you are looking for resolution of all of your components.
Do you know the stationary phase of the column?
Don Shelly wrote:
It's best to choose a highly volatile solvent. The solvent needs to "flash" in the injection port and also precede your analytes through the column so as not to interfere with the resolution of your analytes. That's why I recommended DCM. Essential oils should be miscible in DCM and DCM is very volatile and less likely to be retained by your stationary phase.
I agree with Peter that 10 meters is a very short column if you are looking for resolution of all of your components.
Do you know the stationary phase of the column?
Peter Apps wrote:
Hi Alvin,
How many inlets does it have ? - are both the columns connected to one inlet, or do they go to separate inlets ?
Peter
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