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- Posts: 3
- Joined: Fri Oct 30, 2015 10:57 am
- Location: Barcelona, Spain
I'm working with solid-state fermentation of agricultural residues (like sugarcane bagasse) and I need to quantify the L-phenylalanine content in those residues. I'm able to set up and optimize a HPLC method to do so, but I'm not such confident with the sample preparation step. What I decided to do was a solid-liquid extraction using water as solvent and a further filtration through a 0.45um membrane. Have any of you deal with a similar matrix? The point is that with this simple extraction, some interferences seems to be present very close to the elution point for L-Phe and I'm not sure about the recovery of the process.
Any suggestion would be very helpful
Thanks,