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- Posts: 2
- Joined: Wed Oct 26, 2016 9:56 pm
I am currently only spiking water with thiamine standard.
Here is the current method:
1. Condition Cartridge: 5 mL water, followed by 5 mL methanol.
2. Sample is adjusted to pH 8 and loaded onto cartridge at 1ml/min. (About a quarter of the thiamine is breaking through).
3. Wash with 5 mL of Methanol (No Thiamine).
4. Elute with 0.1% TFA in Methanol. (No Thiamine coming off).
How do I get all of the thiamine to stick to the cartridge?
How do I get all of the thiamine off the cartridge?