standard preparation

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

6 posts Page 1 of 1
Hello,
I have a stock solution concentration of 1 mg/ml for 18 individual standards. i need to prepare a mixture of standards of these 18 stds where the compounds need to be mixed at a concentration of 10 microgram/ml and i need a final volume of 10 ml of this mix solution. Could somebody please elaborate the calculation. Thanks.
10 microgram per ml equals .01 mg/ml.

Multiply .01 times FV of 10 ml = 0.1 mg

0.1 mg times 1 mg/ ml = 0.1 ml or 100 microliters
Don Shelly
LGC Standards
Hi Don,
Thanks for the reply. So if i add 100 microlitres of 1 mg/ml of all the 18 stds into a tube i will get final mixture concentration of 10 microgram/ml. Is that right?

Regards,
Sri
sriharshabio wrote:
Hi Don,
Thanks for the reply. So if i add 100 microlitres of 1 mg/ml of all the 18 stds into a tube i will get final mixture concentration of 10 microgram/ml. Is that right?

Regards,
Sri


and take to a 10 ml final volume with solvent.
Don Shelly
LGC Standards
ok thank You

Regards,
Sri
Hi

Another way of expressing it is

1mg/ml = 1x10-3g/ml = 1x10-4g/100ul

100ul diluted to 10ml
=1x10-4g in 10ml
=1x10-5g in 1ml
0r 10x10-6 in 1ml
=10ug/ml

So yes, 100uL of each - a total 1.8mls made up 10mls will give 10ug/ml

But you really should be capable of working this out for yourself :-)

Regards

Ralph
Regards

Ralph
6 posts Page 1 of 1

Who is online

In total there is 1 user online :: 0 registered, 0 hidden and 1 guest (based on users active over the past 5 minutes)
Most users ever online was 1117 on Mon Jan 31, 2022 2:50 pm

Users browsing this forum: No registered users and 1 guest

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry