Sonication for plasma sample prep

Discussions about sample preparation: extraction, cleanup, derivatization, etc.

3 posts Page 1 of 1
My company inherited a very fancy sonicator (specifically the Q800R2 from QSonica http://www.sonicator.com/19-q800r2-sonicator.html) that we are thinking of bringing into our workflow.

The vast majority of our samples we process are small molecules in plasma. For now, sample prep has been very simple: mixing into acetonitrile, centrifuging, and collecting the supernatant.

Are there any advantages to using the sonicator? Lower LLOQ? Better for other types of fluids or tissue?

Thanks in advance,

Ken
Welcome to the forum.

Rule number one; if it ain't broke, don't fix it.

Sonication only helps if you have immiscible phases.

Peter
Peter Apps
People often sonicate after adding acetonitrile and before centrifugation to help release drugs from plasma proteins. You might make a quick comparison with and without sonication.
3 posts Page 1 of 1

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