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- Posts: 96
- Joined: Thu Dec 16, 2010 3:25 pm
For PP, protein precipitation, I usually use methanol or acetonitrile.
It is said that you have to use a volume (sample:solvent) of 1:1 for acetonitrile and 1:2 for methanol.
Now, I am working on a method where I would be interested on testic acid perchloric as a solvent. I think that in this case you can use lower volumes, but could anybody give me sample:solvent relationship? How lower can I go?
Thank you very much!!!