-
- Posts: 2
- Joined: Thu Nov 19, 2015 5:20 pm
at the moment I am trying to analyse glyphosate, AMPA and glufosinate by the well known derivatization with FMOC-Cl. This reaction is done at pH 9 in a sodium borate buffer solution (7 mM). Unfortunately the reaction is only working well for glyphosate. For glufosinate I get a yield of around 40 % and AMPA is not derivatized at all (at least I see only the underivatized molecule in the mass spectra when tuning for the compounds).
I have to say that this reaction was working before in our institute.
Do you have any idea what could be the reason and what I can try to solve this problem?
Thank you all in advance.