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- Posts: 405
- Joined: Wed Jul 18, 2012 6:45 am
- Location: Germany
There is aquestion that puzzles me at the moment. I want to add an internal standard prior to sample prep to compensate possible loss of volume etc. What happens, when after sample prep the analyte concentration is above the calibrated range? Without ISTD I would dilute the sample and walk off happy, but if I dilute by factor 10, the ISTD concentration will be far away from its normal response - is that still reliable?
The best option would be to dilute the sample prior to sample prep, but I would have to know the result before doing this. Trial and error is no option because sample amount is very limited.
Any idea how to handle this?
Kind regards
Jörg
ps.: I thought about diluting the samples with ISTD solution at the target concentration. It should be possible to compensate this, but will work only if the ISTD extraction efficiency is 100% ?