-
- Posts: 1
- Joined: Mon Nov 24, 2014 4:15 pm
I'm using a Supel™-Select SPE HLB 20 ml tubes to separate hydrophylic and hydrophobic peptides from a peptide mixture sample of 0.1 g diluted in 1 mL of water. In the process of separation we use a vacuum pump to speed up the all process, but it seems not to be working.
I can not find anybody working with these tubes...so Please if you are working with them and even when the question is really simple. Do you use the pump?? maybe we are speeding the process too much? What are the things that can be failing during this separation process?
I would really appreciate all answers
Thanks a lot
PS the protocol we follow (apart from the pump) is the one recomemded
1.100% methanol 2.water 3.sample 4.water 5.10%methanol 6.50 % methanol to collect the second fraction