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- Joined: Mon Jun 16, 2014 7:58 am
In addition to our Shodex Sugar Column we have recently acquired an Aminex HPX-87H in order to view organic acids, mannitol in addition to glucose, maltose and other standard fermentation sugar products.
From the literature available the conditions for running the column have been set:
The eluant is 5mM sulphuric acid
The flow rate is 0.60 ml/min
The temperature is 50 degrees celsius
The detection is UV 210nm.
Using the above conditions, what would be the best sample preparation for running samples and standards:
1)Firstly we are going to adjust the acidic hydrolysate(as a result of the acidic pretreatment phase) to pH 7, using NaOH
2) We are then going to centrifuge
2) We are then going to filter the sample using a 0.45 µm syringe.
Are there any other recommended internal standards/steps in the sample preparation for optimum HPLC separation and viewing.