HFBA derivatization and GC column

[salle]

Hello

I Have a little question about HFBA use and its impact on GC column...
I Explain : I want to reduce the time for amphetamine extraction and derivation. So I would like to remove the step of HFBA neutralisation (with pH 7 buffer) so : put directly the HFBA in my solvants, evaporate them and take back my derivatized analytes with ethyl acetate.... do you think it's a good idea or just silly

thank you for your help

[MSCHemist]

I haven't worked with HFBA but have used HFBI. The only thing you really have to do is add some water and the reagent is quenched and stays in the aqueous layer and the nonpolar fraction is transfered and analyzed. Obviously don't use a wax column otherwise HFBA/HFBI should not react with siloxane based columns.

[mrussell]

I haven't worked with HFBA but have used HFBI. The only thing you really have to do is add some water and the reagent is quenched and stays in the aqueous layer and the nonpolar fraction is transfered and analyzed. Obviously don't use a wax column otherwise HFBA/HFBI should not react with siloxane based columns.

Doesnt restek make a wax column for use with acids.

[MSCHemist]

everyone has FFA columns now adays Agilent, Restek, Phenomenex. The trouble is you either need an automated headspace analyzer or they are a pain to extract from water.

For amphetamines I know I am like a broken record but I love using the chloroformates for derivitization. They work well on amines especially. You can do the reaction in water extract with chloroform and be done in 20 minutes.