-
- Posts: 3
- Joined: Sat May 05, 2012 8:40 am
I found this application note which i am going to follow through.
There is one thing that i can not quite understand. And that is why they are using a Cation Exchange SPE column.. As far as i can see 25-OH D2 and 25-OH D3, are containing Oxygen groups and are hard to ionize to 1+. So how can the vitamin be purified with a cation?
My first thought is to raise the pH and use a Anion exchanger, and lower the pH to eluete my vitamin.
Structure of vitamin D2 and D3:
What do you guys think?
- Adrian