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- Posts: 6
- Joined: Tue Sep 07, 2010 1:11 pm
- Location: United Kingdom
I'm currently extracting total fatty acids from human serum using the traditional folch method. Near the end of the procedure, following esterification with BF3 I reconstitute my samples in hexane and water, vortex and remove the top layer to dry under nitrogen. In about 60% of samples, there will be a red solid residue following drying down- this has only happened since I've been analysing serum samples and does not happen in all samples consistently. It will often happen to one control, but not the other.
Has anyone any ideas as to what could cause a red residue? I'm beginning to think it could be the nitrogen drying- has anyone come across a residue after drying before?
Thanks. Alison