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- Posts: 36
- Joined: Fri Oct 15, 2004 3:57 am
I am trying to pack DAC column (Dynamic axial compression) with regular (10 um) C18 bulk silica gel to scale up a method which was developed in our lab. All my attempts to pack 30x100 mm column failed due to low plate count (3000 plates/meter) and poor symmetry (0.5-0.6). I used some hints from Kromasil (even it is not kromasil silica):
http://www.kromasil.com/kromasil/opencm ... t/packing/
We see over 50K plates/meter on analytical column, so I was expecting at least 30K plates/meter on the prep column, but I am nowhere close. My silica, frits and tubing are new, so I have no idea what is wrong. Changing slurry composition had no effect on plate count and symmetry
Please help. My deadline is at the end of October and I need to do something fast.